Cy3-dCTP is a directly fluorescently labeled deoxyribonucleotide, where Cy3 is a cyanine fluorescent dye. It is used for direct enzymatic labeling of DNA and cDNA, being incorporated into the extending DNA strand during processes such as reverse transcription, PCR, nick translation, or random primer labeling with the aid of DNA polymerases.
- Directly fluorescently labeled deoxyribonucleotide
- Cyanine fluorescent dye
- Used for direct enzymatic labeling of DNA and cDNA
- Incorporated by DNA polymerases during reverse transcription, PCR, nick translation, or random primer labeling